Preparative-scale high-performance liquid chromatography of omega-3 polyunsaturated fatty acid esters derived from fish oil

Marine triglyceride-derived omega-3 polyunsaturated fatty acid ethyl esters were separated by preparative high-performance liquid chromatography on a 25-μm octadecyl stationary phase using a ternary isocratic mobile phase of acetonitrile-tetrahydrofuran-water (466:233:300, v/v/v). The highest purity...

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Bibliographic Details
Published in:Journal of Chromatography A 1988, Vol.459, p.369-378
Main Authors: Beebe, Janet M., Brown, Phyllis R., Turcotte, Joseph G.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fat industries
fatty acid esters
fish oils
Food industries
Fundamental and applied biological sciences. Psychology
liquid chromatography
polyunsaturated fatty acids
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Summary:Marine triglyceride-derived omega-3 polyunsaturated fatty acid ethyl esters were separated by preparative high-performance liquid chromatography on a 25-μm octadecyl stationary phase using a ternary isocratic mobile phase of acetonitrile-tetrahydrofuran-water (466:233:300, v/v/v). The highest purity first-run fractions obtained were ethyl esters of the major marine polyunsaturates eicosapentaenoic acid (20:5ω3, 97.7%) and docosahexaenoic acid (22:6ω3, 93.7%), and the minor polyunsaturate octadecatetraenoic acid (18:4ω3, 98.1%).
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)82049-5