High-performance size-exclusion chromatographic determination of the potency of biosynthetic human growth hormone products

A new high-performance size-exclusion chromatography method has been developed for the determination of potency of human growth hormone products. This method has been extensively validated and shown to correlate well with the hypophysectomized rat bioassay which has been used traditionally. The meth...

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Bibliographic Details
Published in:Journal of Chromatography A 1988, Vol.435 (2), p.307-318
Main Authors: Riggin, R.M., Shaar, C.J., Dorulla, G.K., Lefeber, D.S., Miner, D.J.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Protein hormones. Growth factors. Cytokines
Proteins
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Summary:A new high-performance size-exclusion chromatography method has been developed for the determination of potency of human growth hormone products. This method has been extensively validated and shown to correlate well with the hypophysectomized rat bioassay which has been used traditionally. The method is much more precise than the traditional bioassay and thus provides more reliable means of producing consistent biosynthetic human growth hormone batches.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)82190-7