Quantitation of polyamines using thin-layer chromatography and image analysis

Efficient separation of dansylated polyamines can be achieved by thin-layer chromatography (TLC). Quantitation, however, can be laborious because it requires removal of the silica gel and the fluorescing derivative from the glass plates, elution in a suitable solvent, and estimation with a fluoresce...

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Bibliographic Details
Published in:Journal of Chromatography A 1988, Vol.441 (2), p.361-366
Main Authors: Wettlaufer, Scott H., Weinstein, Leonard H.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Intermediary metabolites. Miscellaneous
Other biological molecules
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Summary:Efficient separation of dansylated polyamines can be achieved by thin-layer chromatography (TLC). Quantitation, however, can be laborious because it requires removal of the silica gel and the fluorescing derivative from the glass plates, elution in a suitable solvent, and estimation with a fluorescence spectrophotometer. We report here a relatively simple and rapid method for the quantitation of dansylated polyamines that employs an image analyzer without removal from the glass TLC plates.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)83879-6