Purification on poly(U)—sepharose 4B of human breast cancer cell line T-47D DNA polymerases

The purification of DNA polymerases (RNA-directed DNA polymerases and DNA-directed DNA polymerases) on poly(U)—Sepharose 4B from a breast tumour cell line (T-47D) is reported. The elution of these enzymes was followed in each fraction by activity measurements with the four primer-templates poly(rA)—...

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Bibliographic Details
Published in:Journal of Chromatography A 1991, Vol.539 (2), p.511-516
Main Authors: Bernard, D., Moyret, C., Maurizis, J.C.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Transferases
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Summary:The purification of DNA polymerases (RNA-directed DNA polymerases and DNA-directed DNA polymerases) on poly(U)—Sepharose 4B from a breast tumour cell line (T-47D) is reported. The elution of these enzymes was followed in each fraction by activity measurements with the four primer-templates poly(rA)—oligo(dT) 12-18, poly(dA) oligo(dT) 12-18, poly(rC)—oligo(dG) 12-18 and poly(rCm)—oligo (dG) 12-18. The control of the polymerase purification by chromatography was performed by sodium dodecyl sulphate—polyacrylamide gel electrophoresis of the pooled active enzymatic fractions.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)83962-5