Perfusion chromatography packing materials for proteins and peptides

This paper describes the performance and properties of column packings for very high-speed, high-capacity and high-resolution separations of proteins and peptides by perfusion chromatography. Perfusion of mobile phase through the supports is achieved by using through-pores of 6000–8000 Å. In additio...

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Bibliographic Details
Published in:Journal of Chromatography A 1991-05, Vol.544 (1-2), p.267-279
Main Authors: Afeyan, N.B., Fulton, S.P., Regnier, F.E.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Proteins
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Summary:This paper describes the performance and properties of column packings for very high-speed, high-capacity and high-resolution separations of proteins and peptides by perfusion chromatography. Perfusion of mobile phase through the supports is achieved by using through-pores of 6000–8000 Å. In addition, these large through-pores are combined with smaller (800–1500 Å) pores which provide high-binding surface area. Media produced from poly(styrene-divinylbenzene) having such a pore structure plus a thin, crosslinked polymer coating allow perfusion chromatography to be performed in the reversed-phase, ion exchange, hydrophobic interaction, immobilized metal affinity and bioaffinity modes.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)83991-1