Determination of the volumes of void zones in packed chromatographic columns

In gas chromatography there is a pause in flow when sorbable material enters the packing and is absorbed by the stationary phase. There is a complementary surge in flow when the band of sorbed material leaves the packing. When a band passes a void zone, these effects combine to produce a doublet rel...

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Bibliographic Details
Published in:Journal of Chromatography A 1988, Vol.445 (1), p.37-48
Main Authors: Rathor, Mohammad N., Buffham, Bryan A., Mason, Geoffrey
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Gas chromatographic methods
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Summary:In gas chromatography there is a pause in flow when sorbable material enters the packing and is absorbed by the stationary phase. There is a complementary surge in flow when the band of sorbed material leaves the packing. When a band passes a void zone, these effects combine to produce a doublet relative to the base-line on a recording of flow-rate against time. Such fluctuations can be measured by using a sensitive differential capillary flow meter. A theory is developed that enables the volumes of void zones in packed chromatographic columns to be found by measuring fluctuations in flow-rate observed when bands of sorbable material pass through the void zones. The theory is confirmed by experiment. The volume of a 0.06-cm 3 void zone was estimated to within about 3%.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)84506-4