The use of Sephadex for the concentration of pteridines

For the isolation of small quantities of pteridines from dilute solutions, such as the culture filtrates of Tetrahymena pyriformis, Sephadex G-25 and G-10 (fine) have proved to be of value. Volumes as large as the void volume of the Sephadex column can be used, resulting in the elimination of large...

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Bibliographic Details
Published in:Journal of Chromatography A 1967-01, Vol.31, p.326-336
Main Authors: Dewey, Virginia C., Kidder, G.W.
Format: Article
Language:English
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Summary:For the isolation of small quantities of pteridines from dilute solutions, such as the culture filtrates of Tetrahymena pyriformis, Sephadex G-25 and G-10 (fine) have proved to be of value. Volumes as large as the void volume of the Sephadex column can be used, resulting in the elimination of large amounts of ultraviolet-absorbing material and in at least a two-fold concentration of the pteridines. Rechromatography on Sephadex results in further concentration. Further purification is achieved using ion-exchange celluloses (cationic and anionic). Ion exchange properties of Sephadex as well as its affinity for aromatic and heterocyclic compounds are postulated as being responsible for these effects.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)86080-5