Use of high-performance liquid chromatography in the preparation of flavin adenine dinucleotide analyte conjugates

High-performance liquid chromatography (HPLC) was used for the study of the chemical synthesis of the flavin adenine dinucleotide analyte conjugates. A preparative HPLC method was also developed for the purification of the conjugates directly from the reaction mixture with minimum sample pretreatmen...

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Bibliographic Details
Published in:Journal of Chromatography A 1985-01, Vol.326, p.301-310
Main Authors: Yip, Kin F., Albarella, James P.
Format: Article
Language:English
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Summary:High-performance liquid chromatography (HPLC) was used for the study of the chemical synthesis of the flavin adenine dinucleotide analyte conjugates. A preparative HPLC method was also developed for the purification of the conjugates directly from the reaction mixture with minimum sample pretreatment. The preparations of flavin N 6-(N-6-aminohexyl)adenine dinucleotide and flavin adenine dinucleotide theophylline conjugate are given as examples to illustrate the importance of this method.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)87455-0