Reversed-phase high-performance liquid chromatography of steroid 3-sulfates and the corresponding unconjugated steroids

Simultaneous separation of steroid 3-sulfates and the corresponding free steroids has been accomplished by reversed-phase high-performance liquid chromatography (RP-HPLC). Addition of ammonium sulfate to the aqueous component of the mobile phase results in retention of the steroid sulfates to octade...

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Bibliographic Details
Published in:Journal of Chromatography A 1984-01, Vol.287 (1), p.97-104
Main Authors: Simonian, Michael H., Capp, Michael W.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Other biological molecules
Terpenes, steroids. Hormones
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Summary:Simultaneous separation of steroid 3-sulfates and the corresponding free steroids has been accomplished by reversed-phase high-performance liquid chromatography (RP-HPLC). Addition of ammonium sulfate to the aqueous component of the mobile phase results in retention of the steroid sulfates to octadecyl silica packings byan apparent ion-pairing mechanism without affecting the retention of the free steroids. The retention time of steroid sulfates was dependent on the ammonium sulfate concentration in the mobile phase. Biologically important C 19 Δ 5,3β-hydroxy-steroids and corresponding 3-sulfate conjugates or estrogen and estrogen 3-sulfates have been resolved with this RP-HPLC system.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)87677-9