Determination of free DOPA and 3-O-methyl-DOPA in human plasma by high-performance liquid chromatography with electrochemical detection

A procedure was devised for the determination of the unconjugated non-protein-bound fraction of 3,4-dihydroxyphenylalanine (DOPA) and 3-O-methyl-DOPA (3-OMD) in plasma using a reversed-phase liquid chromatographic system coupled with electrochemical detection. Sample preparation involves rapid isola...

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Bibliographic Details
Published in:Journal of Chromatography A 1991-08, Vol.553, p.265-269
Main Authors: Melzi D'eril, G.V., Rizzo, V.
Format: Article
Language:English
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Summary:A procedure was devised for the determination of the unconjugated non-protein-bound fraction of 3,4-dihydroxyphenylalanine (DOPA) and 3-O-methyl-DOPA (3-OMD) in plasma using a reversed-phase liquid chromatographic system coupled with electrochemical detection. Sample preparation involves rapid isolation of the unbound drugs from the drug—protein complex by ultrafiltration through a membrane with a molecular weight cut-off of 10 000 dalton. One chromatographic run requires less than 10 min. The relative standard deviation is < 3% for the within-assay imprecision and < 4% for the between-assay imprecision. The detection limits for DOPA and 3-OMD are 0.2 and 1.3 ng/ml, respectively.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)88497-1