Separation of water- and fat-soluble vitamins by micellar electrokinetic chromatography

A mixture of seven water- and two fat-soluble vitamins was successfully separated simultaneously using micellar electrokinetic capillary chromatography. In addition to sodium dodecyl sulphate, modifiers such as γ-cyclodextrin, β-cyclodextrin, and isopropyl alcohol were introduced into the electropho...

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Bibliographic Details
Published in:Journal of Chromatography A 1991-06, Vol.547 (1/2), p.419-428
Main Authors: Ong, C.P., Ng, C.L., Lee, H.K., Li, S.F.Y.
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
chromatography
Exact sciences and technology
fat soluble vitamins
Other chromatographic methods
separation
water-soluble vitamins
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Summary:A mixture of seven water- and two fat-soluble vitamins was successfully separated simultaneously using micellar electrokinetic capillary chromatography. In addition to sodium dodecyl sulphate, modifiers such as γ-cyclodextrin, β-cyclodextrin, and isopropyl alcohol were introduced into the electrophoretic media to investigate their effect on the overall separation of the nine vitamins. Amongst these modifiers, the combination of γ-cyclodextrin with sodium dodecyl sulphate in the electrophoretic medium was found to provide the best selectivity for separating vitamins.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)88665-9