Simultaneous determination of neutral and amino sugars in biological materials

A method is described for the simultaneous analysis of nine neutral and three amino sugars. Mixtures of standard sugars and biological samples were acid hydrolyzed with a two step Saeman procedure, neutralized with BaCO 3, reduced with sodium borohydride, acidified, evaporated and alditol acetates p...

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Bibliographic Details
Published in:Journal of Chromatography A 1990, Vol.513, p.71-81
Main Authors: Kraus, Richard J., Shinnick, Fred L., Marlett, Judith A.
Format: Article
Language:English
Subjects:
amino sugars
determination
dietary fiber
gas chromatography
neutral sugars
oat bran
oats
wheat bran
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Summary:A method is described for the simultaneous analysis of nine neutral and three amino sugars. Mixtures of standard sugars and biological samples were acid hydrolyzed with a two step Saeman procedure, neutralized with BaCO 3, reduced with sodium borohydride, acidified, evaporated and alditol acetates prepared. Baseline resolution was achieved on a glass-capillary SP-2340 column in ca. 52 min. Reproducibility, response factors and hydrolysis losses were determined. Quantitation was linear over the range of 10–20 μg/ml to 2000 μg/ml. Conditions were defined for the reproducible quantitation of muramic acid.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)89426-7