Sensitivity considerations for large molecule detection by capillary electrophoresis-electrospray ionization mass spectrometry

The use of the electrospray ionization (ESI) method for interfacing capillary electrophoresis with mass spectrometry (CE-MS) is particularly well suited for the analysis of large molecules due to the multiple charging phenomenon. While ionization efficiency is very high, the available ion current is...

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Bibliographic Details
Published in:Journal of Chromatography A 1990-09, Vol.516 (1), p.157-165
Main Authors: Smith, Richard D., Loo, Joseph A., Edmonds, Charles G., Barinaga, Charles J., Udseth, Harold R.
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Other chromatographic methods
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Summary:The use of the electrospray ionization (ESI) method for interfacing capillary electrophoresis with mass spectrometry (CE-MS) is particularly well suited for the analysis of large molecules due to the multiple charging phenomenon. While ionization efficiency is very high, the available ion current is dispersed over more peaks so that the maximum peak intensity obtainable declines significantly for large molecules. Sensitivity with ESI can be improved by operation at very low flow-rates, an ideal situation for CE-MS. These and other considerations related to sensitivity are illustrated using ESI-MS measurements for cytochrome c.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)90213-4