High-performance gel filtration of water-soluble samples on polyvinyl alcohol columns

• Published in: Journal of Chromatography A 1985, Vol.320 (2), p.369-377
• Main Authors: Wada, Hiroo, Makino, Keisuke, Takeuchi, Tamio, Hatano, Hiroyuki, Noguchi, Kohji
• Format: Article
• Language: English
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(01)90515-1

The chromatographic behaviour in aqueous solution of pullulans, polyethylene glycols, peptides and proteins with different molecular weights on newly developed polyvinyl alcohol columns (Asahipak GS column series: GS-310, GS-320, GS-510 and GS-520) has been investigated. Pullulans and polyethylene glycols eluted according to the gel filtration mode, so both series of compounds produced almost equal calibration curves for GS-310 and GS-320 and for GS-510 and GS-520; the exclusion limits on GS-310 and GS-320 and on GS-510 and GS-520 were found to be ca. 40,000 and 300,000, respectively. Peptides and proteins were found to adsorb slightly on the columns. However, the plots of the elution volumes against the logarithm of the molecular weights for many of the substrates tested gave rise to the linear calibration curves. Recovery of several crude proteins was also studied for the columns and high values were obtained (81–100%). The effects of changes in the flow-rate, temperature and concentration of electrolytes added to the eluents, on the retention of all four types of compound are also reported.