Column chromatography of nucleotides over thymidylatecellulose

The synthesis of thymidylate-cellulose was accomplished through condensation of 5′-thymidylate and cellulose using dicyclohexylcarbodiimide in pyridine. The thymidylate-cellulose was shown to selectively retain adenosine, 5′-adenylate, and polyadenylate compounds under conditions of high salt concen...

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Bibliographic Details
Published in:Journal of Chromatography A 1966, Vol.21, p.419-423
Main Authors: Sander, Eugene G., McCormick, Donald B., Wright, Lemuel D.
Format: Article
Language:English
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Summary:The synthesis of thymidylate-cellulose was accomplished through condensation of 5′-thymidylate and cellulose using dicyclohexylcarbodiimide in pyridine. The thymidylate-cellulose was shown to selectively retain adenosine, 5′-adenylate, and polyadenylate compounds under conditions of high salt concentration and low temperature which favor hydrogen bonding of the complementary base. Increased temperature was effective during elution of polyadenylate and yeast soluble nucleic acid. Acid hydrolysis was used to cause extensive cleavage of polynucleotides to form mononucleotides, thus indicating the differences in retention on thymidylate-cellulose of polymers as opposed to monomers.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)91335-4