Fast reversed-phase high-performance liquid chromatographic determination of nucleotides in red blood cells

A method for the accurate determination of ATP, ADP, AMP, NADP and NAD in 20 min in red blood extracts by reversed-phase high-performance liquid chromatography is described. Experimental parameters affecting the separation are discussed.

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Bibliographic Details
Published in:Journal of Chromatography A 1984-05, Vol.290, p.393-399
Main Authors: Crescentini, G., Stocchi, V.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Nucleic acids
Nucleic bases, nucleotides
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Description
Summary:A method for the accurate determination of ATP, ADP, AMP, NADP and NAD in 20 min in red blood extracts by reversed-phase high-performance liquid chromatography is described. Experimental parameters affecting the separation are discussed.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)93592-7