Combined monitoring of UV absorbance and fluorescence intensity as a diagnostic criterion in reversed-phase high-performance liquid chromatographic separations of natural phenolic acids

Under standardized conditions, the combined montoring of UV absorbance ( A 280) and fluorescence intensity ( I F) at 390 or 393 nm (on excitation at 300 or 340 nm, respectively) provides a diagnostic criterion for the identification of cinnamic and benzoic acid derivatives by reversed-phase high-per...

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Bibliographic Details
Published in:Journal of Chromatography A 1989, Vol.467 (1), p.149-157
Main Authors: Delcour, J.A., Vinkx, C.J.A., Vanhamel, S., Block, G.G.A.G.
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
Methods of analysis, processing and quality control, regulation, standards
Other chromatographic methods
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Summary:Under standardized conditions, the combined montoring of UV absorbance ( A 280) and fluorescence intensity ( I F) at 390 or 393 nm (on excitation at 300 or 340 nm, respectively) provides a diagnostic criterion for the identification of cinnamic and benzoic acid derivatives by reversed-phase high-performance liquid chromatographic separations of natural phenolic acids. The method can easily be used to determine the concentrations of these compounds if 3,5-dinitrobenzoic acid is applied as an internal standard. When applied to a saponified rye kernel water extract, ferulic, p-coumaric, sinapic, syringic and vanillic acids were shown to be present.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)93959-7