Semi-preparative high-performance reversed-phase displacement chromatography of insulins

A reversed-phase displacement chromatographic method has been developed for the purification of bovine and porcine insulin samples. Up to 500 mg of raw insulin could be purified on a Nucleosil C 8 analytical column using a methanol-containing phosphate buffer carrier and a cetrimide-containing displ...

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Bibliographic Details
Published in:Journal of Chromatography A 1987, Vol.386, p.353-362
Main Authors: Vigh, Gy, Varga-Puchony, Z., Szepesi, G., Gazdag, M.
Format: Article
Language:English
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Summary:A reversed-phase displacement chromatographic method has been developed for the purification of bovine and porcine insulin samples. Up to 500 mg of raw insulin could be purified on a Nucleosil C 8 analytical column using a methanol-containing phosphate buffer carrier and a cetrimide-containing displacer. A proinsulin contamination level as low as 100 ppm in the collected fractions could be achieved.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)94612-6