High-performance liquid chromatographic separation of xanthomegnin and viomellein

Preliminary separations of xanthomegnin and viomellein demonstrated some irreversible binding to silica. However, reversed-phase high-performance liquid chromatographic procedures on C18 columns showed that binding could be minimized by the inclusion of sodium dodecyl sulfate in the mobile phase. Ca...

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Bibliographic Details
Published in:Journal of Chromatography A 1983, Vol.268 (3), p.461-468
Main Authors: Wall, J.H., Lillehoj, E.B.
Format: Article
Language:English
Subjects:
Aminoacids, peptides. Hormones. Neuropeptides
Analytical, structural and metabolic biochemistry
Aspergillus
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Penicillium
Proteins
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Summary:Preliminary separations of xanthomegnin and viomellein demonstrated some irreversible binding to silica. However, reversed-phase high-performance liquid chromatographic procedures on C18 columns showed that binding could be minimized by the inclusion of sodium dodecyl sulfate in the mobile phase. Capacity factors for xanthomegnin and viomellein in several solvent systems are reported. Baseline resolution of the two mycotoxins was achieved with water—acetonitrile—sodium dodecyl sulfate—sulfuric acid (55:45:0.02:0.04, v/v/w/w). Utilization of radial compression technology provided for separation times of 14–16 min. Quantitative techniques were established for the substances based on solvent extraction from the natural substrate followed by reversed-phase high-performance liquid chromatographic separation. In addition to effective separation, the procedure appeared to partially resolve two isomeric forms of viomellein. The toxin occurred in a single form in the initial, crude fungal extract but apparently the compound was racemized during exposure to silica.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)95444-5