Measurement of lipophilicity indices by reversed-phase high-performance liquid chromatography: comparison of two stationary phases and various eluents

Twenty-eight benzene derivatives spanning a broad range of lipophilicities were used as model compounds to examine the optimum stationary phase and eluent conditions for the determination of lipophilic indices by reversed-phase high-performance liquid chromatography. This was assessed by linear regr...

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Bibliographic Details
Published in:Journal of Chromatography A 1991, Vol.541 (1-2), p.221-229
Main Authors: Bechalany, Antoine, Tsantili-Kakoulidou, Anna, El Tayar, Nabil, Testa, Bernard
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Other chromatographic methods
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Summary:Twenty-eight benzene derivatives spanning a broad range of lipophilicities were used as model compounds to examine the optimum stationary phase and eluent conditions for the determination of lipophilic indices by reversed-phase high-performance liquid chromatography. This was assessed by linear regressions comparing published octanol-water partition coefficients with isocratic capacity factors and capacity factors extrapolated to 100% water in the eluent. Methanol-water eluents are always to be preferred to acetonitrile-water and tetrahydrofuran-water eluents. The octadecylsilane (ODS) pahse yielded good correlations especially when a masking agent was added to the eluent, but this introduced an additional experimental variable. The octadecyl-polyvinyl copolymer (ODP) phase was just as satisfactory as the ODS phase without the need for a masking agent, and thus appears to be a valuable alternative.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)95994-1