Separation, isolation and characterization of the four monoiodinated insulin tracers using reversed-phase high-performance liquid chromatography

Baseline separation between insulin and insulin monoiodinated in Tyr A14, A19, B16 and B26 can be obtained using isocratic elution from a C 18 column with triethylammonium trifluoroacetate—acetonitrile and the iodinated insulin derivatives can be isolated by lyophilization. Compared with similar tra...

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Bibliographic Details
Published in:Journal of Chromatography A 1983, Vol.265, p.301-309
Main Authors: S. Welinder, Benny, Linde, Susanne, Hansen, Bruno
Format: Article
Language:English
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Summary:Baseline separation between insulin and insulin monoiodinated in Tyr A14, A19, B16 and B26 can be obtained using isocratic elution from a C 18 column with triethylammonium trifluoroacetate—acetonitrile and the iodinated insulin derivatives can be isolated by lyophilization. Compared with similar tracers purified and isolated by disc electrophoresis/ion-exchange chromatography, the reversed-phase high-performance liquid chromatographically purified tracers are more homogeneous but show reduced binding affinity to adipocytes.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)96725-1