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Chromatographic analysis of photosensitizing dyes

Simple one-phase solvent systems were developed which are useful for the chromatography of a variety of water-soluble acid and alkaline dyes. Using these solvents the purity of several commercial preparations of thiazine, azine, acridine, and xanthene dyes was assayed, and it was shown that such pre...

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Bibliographic Details
Published in:Journal of Chromatography A 1966-01, Vol.24, p.131-140
Main Authors: Bellin, Judith S., Ronayne, Michael E.
Format: Article
Language:English
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Summary:Simple one-phase solvent systems were developed which are useful for the chromatography of a variety of water-soluble acid and alkaline dyes. Using these solvents the purity of several commercial preparations of thiazine, azine, acridine, and xanthene dyes was assayed, and it was shown that such preparations can contain up to sixty percent of isomeric contaminants. These findings point to the need for purifying dye samples before their use in determining absolute quantum yields of photoreduction or photoxidation; spectral changes in absorption due to dye-binding, and spectroscopic properties.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)98111-7