Analysis of basic pharmaceuticals by capillary electrophoresis in coated capillaries and on-line mass spectrometric detection

Basic pharmaceuticals were separated using capillary electrophoresis (CE) and positively identified with the aid of an on-line coupled sectorfield mass spectrometer (MS). The coupling device consisted of a modified electrospray (ES) ion source with coaxial sheath liquid. The adaptation of the CE-con...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 1996-11, Vol.752 (1), p.271-277
Main Authors: Belder, Detlev, Stöckigt, Detlef
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Ephedrine
General pharmacology
Mass spectrometry
Medical sciences
Nozzle-skimmer dissociation
Pharmacology. Drug treatments
Poly(ethylene glycol)
Poly(vinyl alcohol)
Tocainide
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Basic pharmaceuticals were separated using capillary electrophoresis (CE) and positively identified with the aid of an on-line coupled sectorfield mass spectrometer (MS). The coupling device consisted of a modified electrospray (ES) ion source with coaxial sheath liquid. The adaptation of the CE-conditions for the particular needs of ES-MS is discussed in brief. Applying volatile buffers of low ionic strength and using uncoated capillaries in CE causes limited sample loadability, poor peak shapes and decreases resolution. The separation performance can be significantly improved if the capillaries are coated with poly(ethylene glycol) (PEG) or poly(vinyl alcohol) (PVA). As extracted from the reconstructed ion current of the mass spectra, the baselineseparated signals of structurally very similar compounds can be achieved. Signals due to the protonated and Na +-attached molecules have been observed. Nozzle-skimmer dissociation may be a versatile tool for additional molecular structure information.
ISSN:0021-9673
DOI:10.1016/S0021-9673(96)00511-0