Determination of the content and the composition of the main saponins from Solidago gigantea AIT. using high-performance liquid chromatography

A method is presented that allows the determination of the content and the composition of the saponins from Solidago gigantea. A cleanup procedure using C 18 cartridges yields saponin extracts that can be analysed by HPLC using an internal standard. The combination of HPLC on RP-8 and silica results...

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Bibliographic Details
Published in:Journal of Chromatography A 1996-11, Vol.755 (1), p.133-137
Main Authors: Reznicek, G., Freiler, M., Schader, M., Schmidt, U.
Format: Article
Language:English
Subjects:
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Saponins
Solidago gigantea
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Summary:A method is presented that allows the determination of the content and the composition of the saponins from Solidago gigantea. A cleanup procedure using C 18 cartridges yields saponin extracts that can be analysed by HPLC using an internal standard. The combination of HPLC on RP-8 and silica results in the complete separation of the main saponins and thus the composition can be determined. The good correlation between saponin content and hemolytic activity from different samples is shown.
ISSN:0021-9673
DOI:10.1016/S0021-9673(96)00571-7