Determination of phenoxy acid herbicides in vegetation, utilising high-resolution gel permeation chromatographic clean-up and methylation with trimethylsilydiazomethane prior to gas chromatographic analysis with mass-selective detection

A method for the determination of phenoxy acid herbicides in vegetation samples is described. Macerated samples are extracted with acetone and following filtration and acidification, the herbicides are partitioned into dichloromethane. The herbicides are then cleaned-up using high-resolution gel per...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 1996-12, Vol.755 (2), p.245-250
Main Authors: Rimmer, Duncan A., Johnson, Paul D., Brown, Richard H.
Format: Article
Language:English
Subjects:
Derivatization
Environmental analysis
Pesticides
Phenoxy acid herbicides
Trimethylsilyldiazomethane
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A method for the determination of phenoxy acid herbicides in vegetation samples is described. Macerated samples are extracted with acetone and following filtration and acidification, the herbicides are partitioned into dichloromethane. The herbicides are then cleaned-up using high-resolution gel permeation chromatography before undergoing rapid and efficient methylation using trimethylsilyldiazomethane. The resultant methyl esters are then selectively and sensitively analyzed by gas chromatography with mass selective detection. Silanized glassware was used throughout. The method has been applied to grass samples spiked with four phenoxy acid herbicides; 2,4-D, dichlorprop, MCPA and mecoprop. Recoveries around 100% and reproducibilities of 5% ( n=4) were achieved at two spiking levels, ∼700 and ∼140 μg/kg.
ISSN:0021-9673
DOI:10.1016/S0021-9673(96)00615-2