Quantitative determination of protolichesterinic- and fumarprotocetraric acids in Cetraria islandica by high-performance liquid chromatography

A quantitative reversed-phase HPLC method for the analysis of protolichesterinic- and fumarprotocetraric acids in Cetraria islandica (L.) Ach. is described. The compounds were extracted from dry plant material with acetone. HPLC analysis was performed using a LiChrosorb RP-8 column, isocratic elutio...

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Bibliographic Details
Published in:Journal of Chromatography A 1997-01, Vol.757 (1), p.303-306
Main Authors: GUDJONSDOTTIR, G. A, INGOLFSDOTTIR, K
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Aromatic and heterocyclic compounds
Biological and medical sciences
Cetraria islandica
Fumarprotocetraric acid
Fundamental and applied biological sciences. Psychology
Heterocyclic compounds, pigments
Other biological molecules
Protolichesterinic acid
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Summary:A quantitative reversed-phase HPLC method for the analysis of protolichesterinic- and fumarprotocetraric acids in Cetraria islandica (L.) Ach. is described. The compounds were extracted from dry plant material with acetone. HPLC analysis was performed using a LiChrosorb RP-8 column, isocratic elution and UV detection. The method was applied to the quantification of protolichesterinic- and fumarprotocetraric acids in plant material collected in various locations within Iceland. Results showed protolichesterinic acid content of Cetraria islandica to vary between 0.1–0.5% dry weight and fumarprotocetraric acid content to vary between 2.6–11.5% depending on collection site.
ISSN:0021-9673
DOI:10.1016/S0021-9673(96)00670-X