Indirect liquid chromatographic determination of malathion in formulations, based on the formation of palladium(II)-dimethyldithiosphate complex

High-performance liquid chromatographic methods for the determination of an organophosphorous pesticide have been developed, based on alkaline hydrolysis of malathion to dimethyldithiophosphate (DDTP) followed by complexation with palladium(II). The complex is extracted in chloroform and eluted from...

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Bibliographic Details
Published in:Journal of Chromatography A 1997-01, Vol.758 (1), p.159-162
Main Authors: Khuhawar, M.Y., Channar, A.H., Lanjwani, S.N.
Format: Article
Language:English
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Summary:High-performance liquid chromatographic methods for the determination of an organophosphorous pesticide have been developed, based on alkaline hydrolysis of malathion to dimethyldithiophosphate (DDTP) followed by complexation with palladium(II). The complex is extracted in chloroform and eluted from LiChrosorb Si 100 (5 μm), Hypersil ODS, (3 μm) or YMC Pack ODS (5 μm) column with chloroform or methanol-acetonitrile-water (80:10:20, v/v/v). Detection was by UV at 295 nm. Linear calibration is obtained with 24–120 μg/ml and detection limits of 2.4 μg/ml DDTP. The methods are applied for the determination of malathion in commercial formulations. The separation of copper(II) and palladium(II) complexes of DDTP and selectivity of the reaction of DDTP towards copper(II) and palladium(II) are examined by reversed-phase HPLC.
ISSN:0021-9673
DOI:10.1016/S0021-9673(96)00700-5