Absolute quantitation of lignin pyrolysis products using an internal standard

A simple method to obtain absolute quantitation of lignin pyrolysis fragments by on-line pyrolysis–gas chromatography–mass spectrometry (Py–GC–MS) is proposed. Three compounds were tested as internal standards, i.e. 1,3,5-tri-tert.-butylbenzene, 1,2,4-benzenetricarboxylic acid trimethyl ester and 1,...

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Bibliographic Details
Published in:Journal of Chromatography A 1997-06, Vol.773 (1-2), p.227-232
Main Authors: Bocchini, P, Galletti, G.C, Camarero, S, Martinez, A.T
Format: Article
Language:English
Subjects:
Applied sciences
Exact sciences and technology
Lignin
Lignin and derivatives
Natural polymers
Physicochemistry of polymers
Tributylbenzene
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Summary:A simple method to obtain absolute quantitation of lignin pyrolysis fragments by on-line pyrolysis–gas chromatography–mass spectrometry (Py–GC–MS) is proposed. Three compounds were tested as internal standards, i.e. 1,3,5-tri-tert.-butylbenzene, 1,2,4-benzenetricarboxylic acid trimethyl ester and 1,3,5-trimethoxybenzene. The characteristic of the proposed internal standards is that they vaporize in the hot pyrolysis interface during the 3-min equilibration and focus on the top of the GC column, thus avoiding loss of internal standard due to thermal fragmentation. The linearity and reproducibility of response of the standards over a range of concentrations are reported. 1,3,5-Tri-tert.-butylbenzene was selected as the most appropriate among the tested standards to be used in Py–GC–MS of lignin. The correction factors for the main lignin pyrolysis fragments were calculated and a practical application of the proposed method to the analysis of a wheat straw sample is discussed.
ISSN:0021-9673
DOI:10.1016/S0021-9673(97)00114-3