Electromigration behaviour of DNA molecules at the free electrolyte–polymer solution interface

The velocity at which DNA molecules migrate across an interface between a free solution of an electrolyte and a sieving medium was investigated in capillaries. A model was proposed where the DNA molecules are supposed to be stacked at the interface and then, due to consecutive conformational changes...

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Bibliographic Details
Published in:Journal of Chromatography A 1997-06, Vol.772 (1-2), p.243-253
Main Authors: KLEPARNIK, K, MALA, Z, BOCEK, P
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
DNA
Dna, deoxyribonucleoproteins
Fundamental and applied biological sciences. Psychology
Nucleic acids
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Summary:The velocity at which DNA molecules migrate across an interface between a free solution of an electrolyte and a sieving medium was investigated in capillaries. A model was proposed where the DNA molecules are supposed to be stacked at the interface and then, due to consecutive conformational changes, their velocities increase and reach the value of their effective electrophoretic mobilities in the polymer solution filling the rest of a capillary. This behaviour was shown by measuring the electromigration injection bias and by extrapolation of the migration times in capillaries of different lengths to the zero migration path. The experiments showed an extraordinarily high size-based separation selectivity of the electromigration across the interface, which seems to offer a potential for successful high resolution separation of DNA fragments in capillaries as short as several centimetres.
ISSN:0021-9673
DOI:10.1016/S0021-9673(97)00115-5