Capillary zone electrophoresis for the separation and characterization of humic acids

Capillary zone electrophoresis (CZE) was used for the separation and characterization of humic acids (HAs) of different origin. UV–Vis detection and/or fast scanning of spectra during the separation was applied for the detection. From several background electrolytes (BGEs) studied, it was found that...

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Bibliographic Details
Published in:Journal of Chromatography A 1998-04, Vol.802 (1), p.189-202
Main Authors: Fetsch, D, Havel, J
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Humic acids
Other chromatographic methods
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Summary:Capillary zone electrophoresis (CZE) was used for the separation and characterization of humic acids (HAs) of different origin. UV–Vis detection and/or fast scanning of spectra during the separation was applied for the detection. From several background electrolytes (BGEs) studied, it was found that especially those containing amino acids are the most suitable and led to the separation of HAs into several fractions. The composition and pH of buffers were optimized. Up to 30 fractions were separated using a high boric acid concentration (350 m M) BGE. The results can be used as real “fingerprints” to characterize HAs of different origin.
ISSN:0021-9673
DOI:10.1016/S0021-9673(97)01116-3