Effect of Digitalis lanata matrix composition on the lanatoside C partition coefficient and its consequence on rotation locular counter-current chromatography efficiency

Lanatoside C standard samples had their partition coefficients ( K) determined in dichloromethane-methanol-water (4:4:3), (5:6:4) and (7:13:8), by the shake flask method and the obtained values were similar. On the other hand, K for lanatoside C accomplished by calculation from rotation locular coun...

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Bibliographic Details
Published in:Journal of Chromatography A 1998-09, Vol.822 (1), p.37-44
Main Authors: Braga, Fernão Castro, Kreis, Wolfgang, de Oliveira, Alaíde Braga
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cardenolides
Counter-current chromatography
Digitalis lanata
General pharmacology
Lanatosides
Matrix effects
Medical sciences
Partition coefficients
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
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Summary:Lanatoside C standard samples had their partition coefficients ( K) determined in dichloromethane-methanol-water (4:4:3), (5:6:4) and (7:13:8), by the shake flask method and the obtained values were similar. On the other hand, K for lanatoside C accomplished by calculation from rotation locular counter-current chromatography (RLCC) traces of Digitalis lanata pre-purified extracts, as well as by the shake flask method, in the same solvent systems, showed a significant variation. Such variation was attributed to differences in the pre-purification procedures for D. lanata extract, which resulted in starting materials for RLCC with very distinct cardenolide profiles, reflecting, thus, in the K values for lanatoside C and, consequently, in the lanatoside C purity at the end of the chromatographic process.
ISSN:0021-9673
DOI:10.1016/S0021-9673(98)00559-7