Frontal chromatography of proteins: The effect on column performance of the restricted diffusion of molecules in porous chromatographic adsorbents

A theoretical formulation is presented that can be used to describe the dynamic behavior of frontal chromatography of proteins in columns packed with adsorbent particles in which restricted pore diffusion of the adsorbate molecules occurs. The results of this work clearly indicate that the time for...

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Bibliographic Details
Published in:Journal of Chromatography A 1998-12, Vol.828 (1), p.345-356
Main Authors: Liapis, A.I., Sadikoglu, H., Crosser, O.K.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Proteins
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Summary:A theoretical formulation is presented that can be used to describe the dynamic behavior of frontal chromatography of proteins in columns packed with adsorbent particles in which restricted pore diffusion of the adsorbate molecules occurs. The results of this work clearly indicate that the time for breakthrough and the effective utilization of the adsorptive capacity of the chromatographic particles increase as (a) the size of the adsorbate and/or ligand (active site) molecule decreases, (b) the pore connectivity, n T, of the porous network of the adsorbent particles increases, and (c) the column length, L, increases.
ISSN:0021-9673
DOI:10.1016/S0021-9673(98)00642-6