Feasibility study of using refrigerant 1,1,1,2-tetrafluoroethane as the mobile phase in high-speed counter-current chromatography

Newly synthesized environmentally benign refrigerant 1,1,1,2-tetrafluoroethane (R134a) was used as the mobile phase in high-speed counter-current chromatography (HSCCC) in this study. R134a remains as a liquid under mild pressure and becomes a gas at atmospheric pressure. Accordingly, operation of H...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 1999-03, Vol.835 (1), p.237-242
Main Authors: Tsai, Ya-Ling, Yu, Tiing
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
General pharmacology
Medical sciences
Other chromatographic methods
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Tetrafluoroethane
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Newly synthesized environmentally benign refrigerant 1,1,1,2-tetrafluoroethane (R134a) was used as the mobile phase in high-speed counter-current chromatography (HSCCC) in this study. R134a remains as a liquid under mild pressure and becomes a gas at atmospheric pressure. Accordingly, operation of HSCCC equipped with regular polytetrafluoroethylene tubing was satisfactory. Since R134a turned into gas at ambient temperature and pressure, further concentration for the collected fractions after elution was not required. A mixture of four standard components of Angelicae radix was separated to demonstrate the feasibility of using this new mobile phase in HSCCC.
ISSN:0021-9673
DOI:10.1016/S0021-9673(98)01059-0