Sample concentration by sample stacking and sweeping using a microemulsion and a single-isomer sulfated β-cyclodextrin as pseudostationary phases in electrokinetic chromatography

Two of the powerful on-line sample concentration techniques, sample stacking and sweeping under pH-suppressed electroosmotic flow, have been evaluated using a microemulsion and a single-isomer sulfated β-cyclodextrin derivative in electrokinetic chromatography. Several clinically relevant steroids h...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 1999-04, Vol.838 (1), p.3-10
Main Authors: Quirino, Joselito P, Terabe, Shigeru, Otsuka, Koji, Vincent, J.Bryan, Vigh, Gyula
Format: Article
Language:English
Subjects:
Pesticides
Phenols
Steroids
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two of the powerful on-line sample concentration techniques, sample stacking and sweeping under pH-suppressed electroosmotic flow, have been evaluated using a microemulsion and a single-isomer sulfated β-cyclodextrin derivative in electrokinetic chromatography. Several clinically relevant steroids have been separated and concentrated using a microemulsion consisting of 100 m M sodium dodecyl sulfate, 41 m M n-heptane and 700 m M 1-butanol in 50 m M phosphoric acid (pH 1.9). Three environmentally relevant phenoxy acid herbicides and their enantiomers have been separated and concentrated using a background electrolyte consisting of 20 m M hepta-6-sulfato-β-cyclodextrin in 15 m M phosphoric acid (pH 1.9). Significant detector response improvements have been achieved and utilized for analysis of a relatively clean matrix, lake water.
ISSN:0021-9673
DOI:10.1016/S0021-9673(99)00235-6