Utilization of microgravity to improve the crystal quality of biologically important proteins: chaperonin-60, GrpE, B-subunit of V-type ATPase, and MIF

Protein crystallization experiments under microgravity were performed by using commercial vapor diffusion apparatus (CVDA) hardware on the STS-84 shuttle mission. Chaperonin-60, GrpE, the B subunit of V-type ATPase, and the selenomethionine substituted proteins of macrophage migration inhibitory fac...

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Bibliographic Details
Published in:Journal of crystal growth 2000-03, Vol.210 (4), p.819-823
Main Authors: Kitano, Ken, Sasaki, Ryo, Nogi, Terukazu, Fukami, Takaaki A, Nakagawa, Atsushi, Miki, Kunio, Tanaka, Isao
Format: Article
Language:English
Subjects:
ATPase
Biological and medical sciences
Chaperonin
Fundamental and applied biological sciences. Psychology
GrpE
In solution. Condensed state. Thin layers
Macrophage migration inhibitory factor
Microgravity
Molecular biophysics
Physico-chemical properties of biomolecules
Protein crystallization
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Summary:Protein crystallization experiments under microgravity were performed by using commercial vapor diffusion apparatus (CVDA) hardware on the STS-84 shuttle mission. Chaperonin-60, GrpE, the B subunit of V-type ATPase, and the selenomethionine substituted proteins of macrophage migration inhibitory factor (MIF) were selected as target proteins. The results indicate that microgravity positively affected their crystal growth, supporting the practical utilization of microgravity for the protein structure determination.
ISSN:0022-0248
1873-5002
DOI:10.1016/S0022-0248(99)00902-1