Single- and countable-molecule detection of non-fluorescent molecules in liquid phase

A thermal lens microscope was applied to ultrasensitive detection of non-fluorescent molecule in liquid. The calibration curve showed good linearity in the 5.4–27 molecules region. The minimum number of molecules detected was 5.4 and the detection limit of Pb(II) octaethylporphyrin in benzene was 0....

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Bibliographic Details
Published in:Journal of luminescence 1999-11, Vol.83, p.261-264
Main Authors: Tokeshi, Manabu, Uchida, Marika, Uchiyama, Kenji, Sawada, Tsuguo, Kitamori, Takehiko
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Exact sciences and technology
General, instrumentation
Non-fluorescent molecule
Single-molecule detection
Thermal lens microscope (TLM)
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Summary:A thermal lens microscope was applied to ultrasensitive detection of non-fluorescent molecule in liquid. The calibration curve showed good linearity in the 5.4–27 molecules region. The minimum number of molecules detected was 5.4 and the detection limit of Pb(II) octaethylporphyrin in benzene was 0.5 molecules. Our thermal lens microscope is a powerful tool for ultrasensitive detection of non-fluorescent molecules at the single-molecule level and offers a great potential for further applications.
ISSN:0022-2313
1872-7883
DOI:10.1016/S0022-2313(99)00109-X