Efficacy of denture cleansers on Candida spp. biofilm formed on polyamide and polymethyl methacrylate resins

• Published in: The Journal of prosthetic dentistry 2011, Vol.105 (1), p.51-58
• Main Authors: de Freitas Fernandes, Frederico Silva, DDS, Pereira-Cenci, Tatiana, DDS, MSc, PhD, da Silva, Wander Jose, DDS, MSc, PhD, Filho, Antonio Pedro Ricomini, DDS, MSc, Straioto, Fabiana Gouveia, DDS, MSc, Del Bel Cury, Altair Antoninha, DDS, MSc, PhD
• Format: Article
• Language: English
• Subjects: Acrylic Resins - chemistry; Biofilms - drug effects; Biofilms - growth & development; Borates - pharmacology; Candida - drug effects; Candida - physiology; Candida albicans - drug effects; Candida albicans - physiology; Candida glabrata - drug effects; Candida glabrata - physiology; Colony Count, Microbial; Dental Disinfectants - pharmacology; Dental Materials - chemistry; Dentistry; Denture Cleansers - pharmacology; Humans; Humidity; Materials Testing; Mycology - methods; Nylons - chemistry; Polymethyl Methacrylate - chemistry; Single-Blind Method; Sodium Hypochlorite - pharmacology; Sulfates - pharmacology; Surface Properties; Temperature; Time Factors; Wettability
• ISSN: 0022-3913; 1097-6841
• DOI: 10.1016/S0022-3913(10)60192-8

Statement of problem Although new materials have emerged as options to fabricate removable dental prostheses, the development of Candida biofilms on these materials and the effectiveness of methods to control these pathogenic biofilms are poorly understood. Purpose The purpose of this study was to evaluate the efficacy of denture cleansers on Candida single- and dual-species biofilms formed on polyamide resin. Material and Methods Polymethyl methacrylate (PMMA) resin (Acron MC) and polyamide resin (Flexite M.P.) specimens (n=116) were prepared, and their surface roughness was standardized (0.34 ±0.02 μm). Surface free energy (SFE) was measured for some specimens (n=20 per resin), while the remainder were randomly divided by lottery into 24 groups (n=8) for biofilm assay. C. albicans and/or C. glabrata biofilm was formed for 72 hours, and then specimens were treated with an enzymatic cleanser solution (Polident 3 Minutes), a cleanser solution (Corega Tabs), or 0.5% sodium hypochlorite (NaOCl) solution. Water served as the negative control. Remaining adherent microorganisms were removed from the treated specimens by ultrasonic waves, and colony-forming units (CFU) of each microorganism were calculated. SFE data were analyzed by 1-way ANOVA, and Candida species data were analyzed by 3-way ANOVA followed by the Tukey-Kramer test ( P =.05). Results All tested biofilms displayed significantly higher growth on polyamide resin ( P