Differential inhibition of progesterone synthesis in bovine luteal cells by estrogens and androgens

We investigated the roles of estrogens and androgens in the progesterone biosynthesis of bovine luteal cells. The responsiveness of primary luteal cells to the stimulation of tropic agents was observed in a dose-dependent manner. Estrogens and androgens significantly inhibited tropic agent-induced p...

Full description

Saved in:
Bibliographic Details
Published in:Life sciences (1973) 2001-03, Vol.68 (16), p.1851-1865
Main Authors: Guo, Ing-Cherng, Wu, Leang-Shin, Lin, Jen-Hsou, Chung, Bon-chu
Format: Article
Language:English
Subjects:
3β-HSD
8-Bromo Cyclic Adenosine Monophosphate - pharmacology
Androgen
Animals
Cattle
Cells, Cultured - metabolism
Cholesterol Side-Chain Cleavage Enzyme - antagonists & inhibitors
Cholesterol Side-Chain Cleavage Enzyme - metabolism
Corpus Luteum - drug effects
Corpus Luteum - metabolism
Dose-Response Relationship, Drug
Enzyme Inhibitors - pharmacology
Enzyme-Linked Immunosorbent Assay
Estradiol - pharmacology
Estrogen
Female
Hydroxysteroid Dehydrogenases - antagonists & inhibitors
Hydroxysteroid Dehydrogenases - metabolism
Immunoblotting
Luteal cell
P450scc
Progesterone
Progesterone - antagonists & inhibitors
Progesterone - biosynthesis
Testosterone - pharmacology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We investigated the roles of estrogens and androgens in the progesterone biosynthesis of bovine luteal cells. The responsiveness of primary luteal cells to the stimulation of tropic agents was observed in a dose-dependent manner. Estrogens and androgens significantly inhibited tropic agent-induced progesterone secretions, but glucocorticoids did not, which indicated the inhibitions were specific. The failure of exogenous 8-Br-cAMP to prevent these inhibitions suggested that took place at the post-cAMP steps. The immunoblot showed that testosterone remarkably decreased the amount of induced P450scc protein after 6-hour treatment, yet 17β-estradiol did not. The 3β-HSD activity assays demonstrated that both 17β-estradiol and testosterone efficiently blocked induced 3β-HSD activities. Both inhibitory effects of E2 and T on progesterone synthesis were observed one hour after treatment and accompanied with suppressed 3β-HSD activities. This study presents that estrogens and androgens specifically inhibit bovine luteal function through different mechanisms.
ISSN:0024-3205
1879-0631
DOI:10.1016/S0024-3205(01)00983-3