Activation of extracellular signal-regulated kinase in lung tissues of mice treated with carcinogen

The activation of extracellular signal-regulated kinase (ERK) in lung tissues of mice, as determined by the appearance of phosphorylated form, was observed on day 30 after urethane injection, and the activation also occurred in urethane-induced lung tumors. Immunohistochemical analysis using anti-ph...

Full description

Saved in:
Bibliographic Details
Published in:Life sciences (1973) 1999, Vol.64 (4), p.229-236
Main Authors: Yano, Tomohiro, Yano, Yoshihisa, Nagashima, Yoji, Yuasa, Mutsuko, Yajima, Shoko, Horikawa, Saburo, Hagiwara, Kiyokazu, Kishimoto, Mikako, Ichikawa, Tomio, Otani, Shuzo
Format: Article
Language:English
Subjects:
alveolar epithelial cell
Animals
Calcium-Calmodulin-Dependent Protein Kinases - metabolism
Carcinogens - pharmacology
Cell Nucleus - metabolism
Enzyme Activation - drug effects
Epithelial Cells - drug effects
Epithelial Cells - enzymology
ErbB Receptors - metabolism
ERK
Genistein - pharmacology
lung carcinogenesis
Lung Neoplasms - chemically induced
Lung Neoplasms - enzymology
Male
MAP Kinase Kinase 1
Mice
Mice, Inbred A
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase Kinases
Mitogen-Activated Protein Kinases
Mutation
Oncogene Protein p21(ras) - genetics
Oncogene Protein p21(ras) - metabolism
Phosphorylation - drug effects
Protein-Serine-Threonine Kinases - metabolism
Protein-Tyrosine Kinases - metabolism
Proto-Oncogene Proteins c-raf - metabolism
Pulmonary Alveoli - drug effects
Pulmonary Alveoli - enzymology
Ras
Signal Transduction - drug effects
urethane
Urethane - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The activation of extracellular signal-regulated kinase (ERK) in lung tissues of mice, as determined by the appearance of phosphorylated form, was observed on day 30 after urethane injection, and the activation also occurred in urethane-induced lung tumors. Immunohistochemical analysis using anti-phosphorylated ERK antibody indicated that the active form of ERK localized in alveolar epithelial cells. Furthermore, we confirmed by immunoprecipitation and immunoblot analysis that other essential components of the ERK cascade, that is, Ras, Raf and MEK (known as ERK kinase) were activated. These results indicate that the activation of the ERK signal in alveolar epithelial cells at the early stage of urethane-induced lung carcinogenesis is an important factor to develop lung tumors.
ISSN:0024-3205
1879-0631
DOI:10.1016/S0024-3205(98)00558-X