Isolation and characterization of Dioclea altissima var. megacarpa seed lectin

A lectin was isolated from Dioclea altissima var. megacarpa seeds by affinity chromatography on Sephadex G-50 column. The lectin showed carbohydrate specificity for d-mannose/ d-glucose, and divalent metal cations (Ca 2+ and Mn 2+ are required for full activity. It contained no covalently bound carb...

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Bibliographic Details
Published in:Phytochemistry (Oxford) 1997-09, Vol.46 (1), p.139-144
Main Authors: Moreira, Renato A., Monteiro, Ana C.O., Horta, Ana C.G., Oliveira, José T.A., Cavada, Benildo S.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
d-mannose ( d-glucose)-binding
Dioclea altissima
Fundamental and applied biological sciences. Psychology
Glycoproteins
lectin
Leguminosae
Proteins
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Summary:A lectin was isolated from Dioclea altissima var. megacarpa seeds by affinity chromatography on Sephadex G-50 column. The lectin showed carbohydrate specificity for d-mannose/ d-glucose, and divalent metal cations (Ca 2+ and Mn 2+ are required for full activity. It contained no covalently bound carbohydrate and had an amino acid composition characterised by large contents of aspartic acid and serine. The activation energy of the heat denaturing process was 106.9 kJ mol −1. The lectin presented three protein bands in SDS-PAGE, with M r of 26 000, 15 000 and 9000, respectively, and showed immunological identity and the same N-terminal sequence as other lectins already isolated from the sub-tribe Diocleinae. © 1997 Elsevier Science Ltd. All rights reserved
ISSN:0031-9422
1873-3700
DOI:10.1016/S0031-9422(97)00262-8