Biogeneration of 1-octen-3-ol by lipoxygenase and hydroperoxide lyase activities of Agaricus bisporus

The biogeneration of 1-octen-3-ol by lipoxygenase and associated hydroperoxide lyase activities of a homogenate of the cultivated mushroom Agaricus bisporus was investigated using linoleic acid as a substrate. The optimal substrate and protein concentrations were 1.5 mM and 1.5 mg ml −1, respectivel...

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Bibliographic Details
Published in:Process biochemistry (1991) 2001-10, Vol.37 (2), p.177-182
Main Authors: Husson, F, Bompas, D, Kermasha, S, Belin, J.M
Format: Article
Language:English
Subjects:
1-octen-3-ol
Agaricus bisporus
Biogeneration
Hydroperoxide lyase
Lipoxygenase
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Summary:The biogeneration of 1-octen-3-ol by lipoxygenase and associated hydroperoxide lyase activities of a homogenate of the cultivated mushroom Agaricus bisporus was investigated using linoleic acid as a substrate. The optimal substrate and protein concentrations were 1.5 mM and 1.5 mg ml −1, respectively. The V max value for the enzymic coupled reaction lipoxygenase–hydroperoxide lyase was 6 μg of 1-octen-3-ol ml −1 min −1, with a K m value of 0.3×10 −3 M. The highest enzymic activity was observed in the presence of pure oxygen (22.9 μg of 1-octen-3-ol ml −1), with a bioconversion yield of 36%. The production of 380 μg 1-octen-3-ol per g of mushroom homogenate was demonstrated using an oxygenated reaction medium of 1-l containing 0.5% polyvinylpyrrolidone.
ISSN:1359-5113
1873-3298
DOI:10.1016/S0032-9592(01)00201-1