Rapid assay for amidohydrolase (urease) activity in environmental samples

The use of microplate technology for enzyme assays has made it economical to measure a wide range of activities in environmental samples. Urease is one of the most widely measured soil enzyme activities, but current methods are cumbersome. We have developed a rapid, safe and sensitive assay that can...

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Bibliographic Details
Published in:Soil biology & biochemistry 2000-12, Vol.32 (14), p.2095-2097
Main Authors: Sinsabaugh, R.L, Reynolds, H, Long, T.M
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Amidohydrolase
Assay method
Biochemistry
Biochemistry and biology
Biological and medical sciences
Chemical, physicochemical, biochemical and biological properties
Fundamental and applied biological sciences. Psychology
Microplate assay
Physics, chemistry, biochemistry and biology of agricultural and forest soils
Soil enzyme assay
Soil science
Urease
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Summary:The use of microplate technology for enzyme assays has made it economical to measure a wide range of activities in environmental samples. Urease is one of the most widely measured soil enzyme activities, but current methods are cumbersome. We have developed a rapid, safe and sensitive assay that can be performed on microplates.
ISSN:0038-0717
1879-3428
DOI:10.1016/S0038-0717(00)00102-4