Activity of glycolytic enzymes in the gut of Hormogaster elisae (Oligochaeta, Hormogastridae)

• Published in: Soil biology & biochemistry 2000-07, Vol.32 (7), p.929-934
• Main Authors: Garvı́n, M.H, Lattaud, C, Trigo, D, Lavelle, P
• Format: Article
• Language: English
• Subjects: Animal and plant ecology; Animal, plant and microbial ecology; Animals; Autoecology; Biological and medical sciences; Earthworms; Enzymes; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; H. elisae; In vitro culture; Invertebrates; Mutualism; Protozoa. Invertebrata
• ISSN: 0038-0717; 1879-3428
• DOI: 10.1016/S0038-0717(99)00222-9

The glycolytic enzymatic activities in the gut of the endogeic earthworm Hormogaster elisae from El Molar (Madrid, Spain) were studied in order to determine its digestive capacity and to assess its alimentary regime. Most endogeic earthworms have weak enzymatic complement and they usually establish mutualistic relationships with soil microflora to digest some organic compounds. Therefore, the intestinal wall tissues were cultured in vitro to assess the origin of the glycolytic enzymes found in the gut and enzymatic activities were measured in both cultured tissues and culture media. H. elisae had a wide but not very strong enzyme complement, since all substrates were degraded but most of them at a low rate. This species cannot produce cellulase and mannanase, so for the digestion of these substrates it probably uses the digestive enzymatic capabilities of the ingested microflora.