Tridodecylamine and its hydrochloride salt: an organo-soluble buffer for subtilisin Carlsberg in non-polar organic solvents

Mixtures of commercially available tridodecylamine and its hydrochloride dissolved in hexane or toluene have been used as acid-base buffers for a suspended immobilised enzyme. The activity of subtilisin Carlsberg increases with the proportion of free amine, to a similar extent in both solvents. This...

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Bibliographic Details
Published in:Tetrahedron letters 2000-05, Vol.41 (21), p.4223-4227
Main Authors: Harper, Neil, Moore, Barry D., Halling, Peter J.
Format: Article
Language:English
Subjects:
amines
enzymes
ionisation
proteins
protonation
solvent effects
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Summary:Mixtures of commercially available tridodecylamine and its hydrochloride dissolved in hexane or toluene have been used as acid-base buffers for a suspended immobilised enzyme. The activity of subtilisin Carlsberg increases with the proportion of free amine, to a similar extent in both solvents. This indicates that the buffer fixes the ionisation state of the enzyme in the range appropriate for catalytic activity.
ISSN:0040-4039
1873-3581
DOI:10.1016/S0040-4039(00)00570-0