Solid-phase DNA binding detection by EPR spectroscopy

We show here that spin probe labeled oligonucleotides coupled with EPR detection can be used to detect oligonucleotide binding, to optimize conditions used for annealing, and provides a highly selective method for oligonucleotide hybridization detection under conditions that model DNA biochips. Grap...

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Bibliographic Details
Published in:Tetrahedron letters 2002-03, Vol.43 (11), p.1931-1933
Main Authors: Gannett, Peter M, Powell, Jeannine H, Johnson, Edward M, Darian, Eva, Dalal, Naresh S, Norton, Michael L, Budil, David E
Format: Article
Language:English
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Summary:We show here that spin probe labeled oligonucleotides coupled with EPR detection can be used to detect oligonucleotide binding, to optimize conditions used for annealing, and provides a highly selective method for oligonucleotide hybridization detection under conditions that model DNA biochips. Graphic
ISSN:0040-4039
1873-3581
DOI:10.1016/S0040-4039(02)00161-2