Tracer studies on dinoflagellate luciferin with [ 15N]-glycine and [ 15N]- l-glutamic acid in the dinoflagellate Pyrocystis lunula

The bioluminescence of dinoflagellate is a typical luciferin–luciferase reaction. To clarify the biosynthesis of dinoflagellate luciferin, we performed a feeding experiment with [ 15N]-glycine and [ 15N]- l-glutamic acid in the dinoflagellate Pyrocystis lunula. In a control experiment, we also exami...

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Bibliographic Details
Published in:Tetrahedron letters 2003-02, Vol.44 (6), p.1263-1266
Main Authors: Wu, Chun, Akimoto, Hidetoshi, Ohmiya, Yoshihiro
Format: Article
Language:English
Subjects:
bioluminescence
biosynthesis
chlorophyll a
dinoflagellate luciferin
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Summary:The bioluminescence of dinoflagellate is a typical luciferin–luciferase reaction. To clarify the biosynthesis of dinoflagellate luciferin, we performed a feeding experiment with [ 15N]-glycine and [ 15N]- l-glutamic acid in the dinoflagellate Pyrocystis lunula. In a control experiment, we also examined whether or not chlorophyll a was incorporated with these labeled compounds. We detected by mass spectrometry the incorporation of [ 15N]-glycine and [ 15N]- l-glutamic acid into the four tetrapyrrole rings of the luciferin. In the control experiment, chlorophyll a was also incorporated with [ 15N]-glycine and [ 15N]- l-glutamic acid. Our results show that either glycine or glutamic acid could be the original component of dinoflagellate luciferin as well as chlorophyll a in the dinoflagellate P. lunula. The bioluminescence of dinoflagellate is a typical luciferin–luciferase reaction. To clarify the biosynthesis of dinoflagellate luciferin, we performed a feeding experiment with [ 15N]-glycine and [ 15N]- l-glutamic acid in the dinoflagellate Pyrocystis lunula. In a control experiment, we also examined whether or not chlorophyll a was incorporated with these labeled compounds. We detected by mass spectrometry the incorporation of [ 15N]-glycine and [ 15N]- l-glutamic acid into the four tetrapyrrole rings of the luciferin. In the control experiment, chlorophyll a was also incorporated with [ 15N]-glycine and [ 15N]- l-glutamic acid. Our results show that either glycine or glutamic acid could be the original component of dinoflagellate luciferin as well as chlorophyll a in the dinoflagellate P. lunula.
ISSN:0040-4039
1873-3581
DOI:10.1016/S0040-4039(02)02815-0