An improved method for the synthesis of N3′→P5′ phosphoramidate oligonucleotides

A new method for the synthesis of N3′→P5′ phosphoramidate oligonucleotides is demonstrated. This phosphoramidite amine-exchange method generates cycle yields in the 92–95% range, and provides overall yields that are 3–6 times higher than the previously published oxidative phosphorylation method. new...

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Bibliographic Details
Published in:Tetrahedron letters 1997-01, Vol.38 (2), p.207-210
Main Authors: McCurdy, Sarah N., Nelson, Jeffrey S., Hirschbein, Bernard L., Fearon, Karen L.
Format: Article
Language:English
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Summary:A new method for the synthesis of N3′→P5′ phosphoramidate oligonucleotides is demonstrated. This phosphoramidite amine-exchange method generates cycle yields in the 92–95% range, and provides overall yields that are 3–6 times higher than the previously published oxidative phosphorylation method. new method for the synthesis of N3′→P5′ phosphoramidate oligonucleotides is demonstrated.
ISSN:0040-4039
1873-3581
DOI:10.1016/S0040-4039(96)02254-X