The DLP1 mutant of the yeast Saccharomyces cerevisiae with an increased copy number of the 2μ plasmid shows a shortened lifespan

• Published in: Mechanisms of ageing and development 1999-10, Vol.110 (1), p.119-129
• Main Authors: Xu, Zhaojun, Mitsui, Kazuhiro, Motizuki, Mitsuyoshi, Yaguchi, So-Ichi, Tsurugi, Kunio
• Format: Article
• Language: English
• Subjects: 2μ plasmid; ARS element; Autophagic death; Biological and medical sciences; Budding yeast; Classical genetics, quantitative genetics, hybrids; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; Lifespan; Nibbled colony; Thallophyta, bryophyta; Vegetals
• ISSN: 0047-6374; 1872-6216
• DOI: 10.1016/S0047-6374(99)00052-4

We isolated and characterized a recessive mutant, named dlp1, which shows the Dlp phenotype (delayed loss of proliferation activity) during the autophagic death of cdc28. The dlp1 mutant was found to consist of two subtypes of cells based on colony morphology. One subtype with the Dlp phenotype, named dlp1-l, became large, red, and nibbled during the incubation, suggesting that the cells on the surface of the colonies were dying. The other without the Dlp phenotype, named dlp1-s, retained small, white colonies even after a prolonged incubation and was found to be a petite mutant. The change from dlp1-l to dlp1-s (petite) occurred much more frequently (about 15%) than that from the wild-type to petite mutant (less than 1%). The lifespan of both subtypes of cells was severely shortened. The copy number of the endogenous 2μ plasmid of dlp1-l was 68-fold that of the original cdc28, and decreased by half after the conversion to dlp1-s (petite). A 4.0-kbp fragment of the 2μ plasmid containing REP2 decreased the copy number of the endogenous 2μ plasmid to 8-fold that of the original cdc28 cells and partially rescued the shortened lifespan, in addition to resulting in the complete complementation of the Dlp and nibbled-colony phenotypes. These results suggest that DLP1 is a chromosomal gene that regulates the copy number of the 2μ plasmid, and that the shortening of the lifespan and other effects of the dlp1 mutation are likely caused by the increased copy number of the endogenous 2μ plasmid.