Variability in platelet responses to collagen — comparison between whole blood perfusions, traditional platelet function tests and PFA-100

The purpose of this study was to determine if the results obtained in platelet function tests and whole blood perfusions are associated with those in platelet function analyser (PFA)-100. We used collagen type I monomers and fibrils to analyse the distinct roles of glycoprotein (GP) Ia/IIa and other...

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Bibliographic Details
Published in:Thrombosis research 2001-07, Vol.103 (2), p.123-133
Main Authors: Lepäntalo, Aino, Beer, Jürg H, Siljander, Pia, Syrjälä, Martti, Lassila, Riitta
Format: Article
Language:English
Subjects:
Adenosine Diphosphate - pharmacology
Adult
Aged
Amino Acid Chloromethyl Ketones - pharmacology
Anticoagulants - pharmacology
Biological and medical sciences
Blood and lymphatic vessels
Blood Platelets - drug effects
Cardiology. Vascular system
Citric Acid - pharmacology
Collagen
Collagen - chemistry
Collagen - pharmacology
Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous
Drug Synergism
Epinephrine - pharmacology
Female
Genetic Variation
Glycoproteins
Humans
Integrins - drug effects
Integrins - physiology
Male
Medical sciences
Middle Aged
Perfusion
Platelet
Platelet Activation - drug effects
Platelet Aggregation - drug effects
Platelet Function Tests - instrumentation
Polymorphism, Genetic
Receptors, Collagen
Reproducibility of Results
Rheology
Stress, Mechanical
Thrombosis
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Summary:The purpose of this study was to determine if the results obtained in platelet function tests and whole blood perfusions are associated with those in platelet function analyser (PFA)-100. We used collagen type I monomers and fibrils to analyse the distinct roles of glycoprotein (GP) Ia/IIa and other collagen receptors in flowing blood under a high shear rate (1600/s) and in aggregation studies. Also, anticoagulation [citrate vs. d-phenylalanyl-1-prolyl-1 arginine chloromethyl ketone (PPACK)] was varied to enhance the functions of GP Ia/IIa, since it has been shown that the cation-poor environment of citrated blood impairs GP Ia/IIa-dependent platelet recruitment. Large interindividual variability (45-fold) was detected in deposition of platelets in whole blood perfusions over collagen monomers, whereas this variation was only fourfold in fibrils. In PFA, this variation was reduced to 2.5-fold. However, platelet deposition on monomers is associated with epinephrine-enhanced PFA ( r=−.49, P
ISSN:0049-3848
1879-2472
DOI:10.1016/S0049-3848(01)00283-3