Expression of the proliferating cell nuclear antigen (PCNA) in the rat thyroid gland after exposure to bromide

Analysis of expression of the proliferating cell nuclear antigen (PCNA) was used to determine the presumed hyperplastic character of morphological changes in the rat thyroid evoked by bromide administration. Male rats fed by a standard diet with determined iodine and bromine content were given potas...

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Bibliographic Details
Published in:Acta histochemica 1997-11, Vol.99 (4), p.391-399
Main Authors: Velický, Jan, Titlbach, Milan, Lojda, Zdeněk, Jelinek, František, Vobecký, Miloslav, Raška, Ivan
Format: Article
Language:English
Subjects:
Animals
bromide
Bromides - toxicity
Epithelium - drug effects
Epithelium - pathology
Goiter - chemically induced
Hyperplasia
Immunohistochemistry
Male
Mitosis
mitotic activity
PCNA assay
Proliferating Cell Nuclear Antigen - biosynthesis
rat
Rats
thyroid gland
Thyroid Gland - drug effects
Thyroid Gland - pathology
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Summary:Analysis of expression of the proliferating cell nuclear antigen (PCNA) was used to determine the presumed hyperplastic character of morphological changes in the rat thyroid evoked by bromide administration. Male rats fed by a standard diet with determined iodine and bromine content were given potassium bromide. Control animals received no bromide. Experimental animals were given 10, 50 or 100 mg Brper 11 drinking water for 16 and 66 days, or 100, 200, 400 mg Br -/l drinking water for 133 days. The thyroids of treated animals showed activation of growth of the epithelial follicular component as well as diffuse and focal microfollicular rearrangement of the parenchyma with higher follicular cells accompanied by a decrease of the amount of colloid even at low bromine concentrations (10-100 mg Br -/l drinking water). Using the PCNA-LI index (PCNA-positive nuclei 100/total number of follicular cell nuclei in the section), immunohistochemical analysis of PCNA in the nuclei of the follicular cells was carried out in parrafin sections. The index was significantly higher in bromide exposed animals (P < 0.01) and correlated well with the histological changes, with bromide concentration and with a increased mitotic activity of the follicular cells. PCNA analysis showed that morphological changes resembling a parenchymatic goitre reflect a microfollicular rearrangement of the thyroid of rats exposed to bromide and have the character of hyperplasia owing to the increased mitotic activity of the follicular epithelium.
ISSN:0065-1281
1618-0372
DOI:10.1016/S0065-1281(97)80032-3